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Thus, White (1941), in contrast to Griffith (1929), found that as much as 5 % of sodium benzoate was necessary to retard the growth of rats fed a 6 % casein diet, although no other toxic symptoms were observed. On the other hand, Totter, Amos, and Keith (1949) obtained a marked growth retardation by adding 3 % sodium benzoate t o a n 18% casein diet and thus substantially confirmed the observations of Griffith (1929). Secondly, the growth of rats fed growthretarding amounts of sodium benzoate was apparently not improved by the addition of serine or threonine (White, 1941), which are now known to be efficient glycine precursors.
The excretion is very slow owing to extensive reabsorption of the bile salts, but after 14 days approximately 90 % has been eliminated in the feces (Siperstein and Chaikoff, 1952). Glycine appears to be of only minor significance in bile salt formation. Although ox bile contains five glycine and only two taurine conjugates, the latter form about, 80 % of the total bile salts (Ahrens and Craig, 1952). It is noteworthy, however, that as far as is known, glycine conjugates of bile acids occur only in mammalian species (Haslewood and Wootton, 1950).
Neuberger, 1949). However, the separation of glycine by ion-exchange chromatography and its subsequent estimation with ninhydrin (Stein, 1953) has given more reliable values. In this work, the average excretion of glycine and serine by adult human subjects was found to be 132 and 43 mg. per day, respectively. In addition, about 800 mg. of glycine and 50 mg. of serine were excreted as peptides or in some other combined form. Earlier values obtained for the excretion of glycine are summarized in Table IV.